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120 New Scotland Avenue Albany, NY, 12208 United Stateshe Applied Genomic Technologies Core provides DNA sequencing, genotyping, and microarray services to research investigators. Below are brief descriptions and links to complete descriptions of all services provided by the core. The microarray services provided utilize Affymetrix GeneChips® or glass slide-based arrays. The laboratory processes and hybridizes samples for Affymetrix high-density oligonucleotide arrays (GeneChips) including expression arrays, resequencing arrays, tiling arrays and genotyping arrays. For glass slide-based approaches, samples are labeled and hybridized and scanned using an Agilent scanner. For further information, visit the Microarray home page or contact Zhen Zhang (518) 474-6992. Genotyping services include a variety of approaches for genotyping, as well as DNA purification and PCR troubleshooting/ optimization services. The types of genotyping analyses offered include Taq-Man-based single nucleotide polymorphism (SNP), fragment analysis and gene copy number variation (gene dosage) analysis using multiplex ligation-dependent probe amplification (MLPA). Human cell line verification by short tandem repeat (STR) testing is also available. For further information, visit the Genotyping home page or contact Melissa Leisner (518) 473-6606. DNA Sequencing can be performed on PCR products, plasmids, cosmids and BACs using ABI 3730XL and ABI3130XL instruments. A Biotage PyroMark ID Pyrosequencing System is also used for short-read sequencing and SNP genotyping. For further information, visit the Sequencing home page or contact Matthew Shudt (518) 474-4200. Next-generation sequencing services are provided using the Ion Torrent PGM (Life Technologies) and MiSeq (Illumina) sequencers. Services are available for amplicon re-sequencing and viral or microbial whole genome sequencing. For further information, visit the Sequencing home page or contact Matthew Shudt (shudt@wadsworth.org) (518) 474-4200.
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