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10425-C MRB IV 2213 Garland Ave. Nashville, TN, 37232-0443 United StatesThe Digital Histology Shared Resource provides large-scale digital archiving and quantitative analysis of histologic, immunohistochemical and immunofluorescence staining of tissue sections and tissue microarrays. The Aperio Versa automated scanning microscope and the Leica SCN400 Slide Scanner deliver solutions for high-resolution imaging in both bright field and fluorescence. Both instruments have high-capacity robotic autoloading (200 slides for the Aperio Versa and 384 slides for the Leica SCN400) making them ideal for large slide cohorts and tissue microarrays. The associated software packages provide complex algorithms for unbiased, automated image analysis and quantitation of immunostaining in both bright field and fluorescence. In addition, these systems can be utilized as a permanent high-resolution solution for those who need archiving of histologic material. The DHSR hosts a convenient, web-based digital slide-viewing environment (Digital Image Hub) for the rapid retrieval, review, annotation and image acquisition of scanned material. This easy to use digital slide box allows researchers to share images among colleagues, both at Vanderbilt and at other institutions, from any web browser while remaining in a digitally secure environment. Built-in conferencing features allow investigators to coordinate multi-site discussions of histologic samples. Expert assistance is offered in planning experiments and processing data in a consistent, objective, and timely manner. The automated imaging and analysis performed in this core saves researchers and staff weeks of tedious work. An additional service offered by the DHSR is the creation of digital archives of critical and irreplaceable tissue samples, a benefit only feasible due to the automated high-resolution imaging of whole 25 mm x 75 mm microscope slides and 50 mm x 75 mm "double" slides. The DHSR offers specialized microscopy assistance in addition to automated slide scanning and analysis, such as colony counting, long-term live imaging and deconvolution. The core is capable of high-resolution automated and semi-automated imaging and quantitation of a wide range of cell culture and tissue samples, from bacterial colonies to oranoids.
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