Braidhurst Industrial Estate Newhut Road Motherwell, ML1 3ST United KingdomRoukenCells: Assay-Ready Cell Lines and Immune Cell Banks for Therapeutic Discovery and Development RoukenCells is the cell product arm of RoukenBio, established to provide drug developers with engineered cell lines and ready-to-use immune cell banks for seamless integration into your in-house assay workflows for therapeutic discovery, screening and preclinical development. Employing RoukenBio’s proprietary platforms, RoukenCells supplies off-the-shelf inducible IndEx-2 cell lines, advanced reporter cell lines and primary immune cell banks, providing researchers with fully characterised, ready-to-use cell products. 1. IndEx-2 Inducible Cell Lines IndEx-2 is RoukenCells’ proprietary inducible expression platform for precisely controlling the surface expression of one or two target antigens of interest. Target expression can be titrated across biologically relevant densities, from low physiological expression through to elevated pathological or tumour-associated levels. Dual-inducible IndEx-2 models use orthogonal control mechanisms, allowing two target antigens to be regulated independently within the same cellular background. Researchers can therefore evaluate multiple antigen-density combinations without introducing the confounding genetic differences encountered when comparing unrelated cell lines. 2. Reporter Cell Lines RoukenCells provides ready-to-use reporter cell lines that convert receptor engagement or intracellular pathway activation into quantitative luminescent signals. These models provide scalable, reproducible readouts for functional screening, mechanism-of-action studies, candidate ranking, neutralisation and blocking assays, potency testing and lot-to-lot comparability. The reporter portfolio covers pathways relevant to T-cell activation, immune checkpoints, Fc effector biology, innate immune signalling, allergy, type 2 inflammation, cytokine biology and receptor pharmacology. 3. Primary Immune Cell Banks Our primary immune cell banks provide cryopreserved, Thaw-&-Go human immune effector cells for functional and co-culture assays. Banked products reduce the time and variability associated with repeatedly sourcing, isolating, expanding and qualifying suitable donor material. Our primary immune cell banks provide cryopreserved, Thaw-&-Go human immune effector cells for functional and co-culture assays. Banked products reduce the time and variability associated with repeatedly sourcing, isolating, expanding and qualifying suitable donor material. 4. Stable Overexpressing Cell Lines RoukenCells supplies stable target-expressing cell lines with defined expression characteristics for screening, binding, selectivity, mechanism-of-action and potency assays. These products provide accessible target-positive cells without requiring customers to undertake time-consuming cell-line generation and qualification internally. 5. Knockout Cell Lines RoukenCells is developing a growing collection of target-specific knockout cell lines. These products provide clean target-negative comparators for confirming target dependency, assessing antibody or therapeutic specificity, investigating mechanism of action and distinguishing on-target activity from background or off-target effects. 6. LocIn Targeted Integration Kits LocIn is Rouken’s proprietary targeted-integration platform for generating stable, reproducible cell populations through recombinase-mediated cassette exchange. LocIn platform cells contain a pre-engineered landing pad at a defined genomic locus selected to support reliable transgene expression. During retargeting, the landing-pad cassette is replaced with the user’s target or gene of interest. This controlled integration approach reduces the variable positional effects associated with random integration and can remove the need for lengthy single-cell cloning and extensive clone screening. LocIn enables the rapid generation of stable, isogenic expression populations or clonal-like pools with consistent genotype-to-phenotype relationships. It is applicable to target overexpression, functional screening and scalable cell-line generation. LocIn Retargeting Kits give researchers the flexibility to generate their own target-expressing models, while LocIn ReTarget Cell Lines provide pre-engineered, assay-ready products. RoukenCells products are supplied cryopreserved and shipped on dry ice using controlled preparation and quality-sign-off procedures. All products are intended for research use only.