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305 Grattan Street Melbourne , VIC, 3000 AustraliaThe Tissue bank is a member of the Victorian Cancer Biobank, providing researchers with ethically collected, high quality human tissue, blood and data samples for their investigative projects; it also supports clinical trials at Peter Mac by processing and storing blood and tissue specimens in accordance with trial-specific protocols. HUMAN TISSUE SAMPLES After consenting patients, Peter Mac’s Tissue bank takes annotated specimens of human tissue that are not required for pathological testing. These samples are then "snap frozen" to ensure that ice crystals do not distort the arrangement of cells, and formalin fixed and paraffin embedded (FFPE). The embedded tissue is then sliced into very thin sections. These samples are divided, labelled and grouped for use in research studies, classified by tumour type and subtype, and as cancerous or benign. MICROARRAYS The Tissue bank also prepares tissue microarrays (TMAs), by taking a core ("punching" a hole) through layers of a single FFPE tissue block, across many different sample blocks and combining the cores in one TMA block. Thin sections are then cut from this TMA block, allowing researchers to analyse for a particular gene expression from many different samples in a single experiment, quickly and efficiently. BLOOD SAMPLES Blood samples are collected from consenting donors and separated and stored as mononuclear cells, buffy coat, plasma and serum. The Tissue bank also extracts DNA from tissue and blood products, allowing researchers to look at DNA derived from tumour tissue to identify changes that occur in cancer. They can also look at germline DNA, from blood samples or normal tissue, to determine if a cancer is inherited or acquired.
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