Plesmanlaan 121 Amsterdam, 1066 CX NetherlandsThe Facility provides assessment of protein composition of a variety of samples. Preferably, researchers first discuss with us the project(s) they have coming up, in order to design the optimal experimental setup allowing successful analysis by liquid chromatography-coupled tandem mass spectrometry (LC-MS/MS). For quantitative proteomics experiments, stable isotope labeling at the peptide or protein level can be considered. After performing the experiment, researchers hand in their samples, which we process further for LC-MS/MS. Sample processing involves protein extraction, reduction, alkylation, proteolytic digestion, (optional) multiplex stable isotope labeling and desalting. Finally, peptides are separated by nanoflow reversed-phase liquid chromatography, and analyzed by tandem mass spectrometry. After mass spectrometry analysis, MS/MS spectra are processed with dedicated software to yield protein identification (and quantification, if applicable). Results are exported as Excel workbook and shared with the researcher. Limited bioinformatics analysis can be discussed for further data interpretation. What we take: cell pellets or cell lysates; protein fractions; immunoaffinity beads or eluates from immunoprecipitations; SDS-PAGE gels*. Other sample types? Come and discuss the possibilities! *We prefer to run SDS-PAGE gels ourselves and cut gel bands here at the Facility, so if you're interested in analyzing specific bands, we'd like you to provide protein samples rather than a gel. What we offer: Quantitative & Qualitative (full) proteome analysis; Phosphoproteome analysis; Ubiquitinated proteome analysis Other requests? Come and discuss the possibilities! Targeted protein quantitation (parallel reaction monitoring). Internal projects are on a collaborative basis and free of charge, fitting within the capacity and scope of the facility. However, certain expensive reagents (kits, antibodies etc.) may have to be purchased by the researcher.

Plesmanlaan 121 1066 CX AmsterdamThe Facility provides assessment of protein composition of a variety of samples. Preferably, researchers first discuss with us the project(s) they have coming up, in order to design the optimal experimental setup allowing successful analysis by liquid chromatography-coupled tandem mass spectrometry (LC-MS/MS). For quantitative proteomics experiments, stable isotope labeling at the peptide or protein level can be considered. After performing the experiment, researchers hand in their samples, which we process further for LC-MS/MS. Sample processing involves protein extraction, reduction, alkylation, proteolytic digestion, (optional) multiplex stable isotope labeling and desalting. Finally, peptides are separated by nanoflow reversed-phase liquid chromatography, and analyzed by tandem mass spectrometry. After mass spectrometry analysis, MS/MS spectra are processed with dedicated software to yield protein identification (and quantification, if applicable). Results are exported as Excel workbook and shared with the researcher. Limited bioinformatics analysis can be discussed for further data interpretation. What we take: cell pellets or cell lysates; protein fractions; immunoaffinity beads or eluates from immunoprecipitations; SDS-PAGE gels*. Other sample types? Come and discuss the possibilities! *We prefer to run SDS-PAGE gels ourselves and cut gel bands here at the Facility, so if you're interested in analyzing specific bands, we'd like you to provide protein samples rather than a gel. What we offer: Quantitative & Qualitative (full) proteome analysis; Phosphoproteome analysis; Ubiquitinated proteome analysis Other requests? Come and discuss the possibilities! Targeted protein quantitation (parallel reaction monitoring). Internal projects are on a collaborative basis and free of charge, fitting within the capacity and scope of the facility. However, certain expensive reagents (kits, antibodies etc.) may have to be purchased by the researcher.