Tokyo, JapanHybrigenics Services is a complete service provider of cutting-edge technologies dedicated to the study of proteins and their interactions in 35+ organisms and any research thematic. We offer three main lines of services: • Interactions Discovery: ULTImate Y2H™ to discover novel protein-protein interactions in various tissues/cell types o Several adapted versions allow to identify interactions with transmembrane proteins, DNA and RNA o Associated services: Interaction Domain Mapping, Low binders, super binders • Drug Discovery Services (Adapted from ULTImate Y2H) o Target deconvolution: ULTImate YChemH™ to identify on- and off-targets of small bioactive molecules - mechanism of action, polypharmacology, drug repositioning, prediction of toxicity o Protein-protein interactions induced by molecular glues (protein stabilization, targeted protein degradation) • Nanobody® (or Synthetic Antibodies, VHH) to select and validate high-affinity single-domain antibodies (Hybribody™) - for IF, ELISA, high-resolution microscopy, video-microscopy, target inhibition applications but also for diagnosis and therapeutic purposes Our optimized screening platform in yeast enables the identification of the protein partners of proteins, DNA sequences, RNA and small molecules of therapeutic interest. Highly complex protein domain libraries, prepared from any cell type or tissue, are screened to saturation to identify partners and their interacting domains using a transcriptional read-out. Sophisticated bioinformatics analysis allows to attribute confidence scores to each interaction. With more than 3,200 academic and industrial customers from over 40 countries, Hybrigenics Services is internationally recognized. Among our services, our yeast two-hybrid screening technology, ULTImate Y2H, is extensively used in many research projects. The results obtained at Hybrigenics have been quoted in more than 480 scientific publications to date. Hybribody offers single-domain antibody selection and validation services. We handle the in vitro selection of antibodies against your protein from a fully synthetic humanized VHH library by an optimized Phage Display technology. VHHs have the advantages of being very small (15 kDa), affine and stable molecules. We validate the selected VHHs for in vitro and in vivo applications (ELISA, IF, Intrabody…). We can also fuse the VHH with Fc fragments from the species of your choice to deliver fully functional recombinant antibodies. In addition, Hybrigenics Services provides optimization of nanobodies coming from the Llama using yeast display technology. Nanobody® is a trademark of Ablynx.

1 Rue Pierre Fontaine Genopole Biocluster Campus 3 Évry, Île-de-France, 91058 FranceHybrigenics Services is a complete service provider of cutting-edge technologies dedicated to the study of proteins and their interactions in 35+ organisms and any research thematic. We offer three main lines of services: • Interactions Discovery: ULTImate Y2H™ to discover novel protein-protein interactions in various tissues/cell types o Several adapted versions allow to identify interactions with transmembrane proteins, DNA and RNA o Associated services: Interaction Domain Mapping, Low binders, super binders • Drug Discovery Services (Adapted from ULTImate Y2H) o Target deconvolution: ULTImate YChemH™ to identify on- and off-targets of small bioactive molecules - mechanism of action, polypharmacology, drug repositioning, prediction of toxicity o Protein-protein interactions induced by molecular glues (protein stabilization, targeted protein degradation) • Nanobody® (or Synthetic Antibodies, VHH) to select and validate high-affinity single-domain antibodies (Hybribody™) - for IF, ELISA, high-resolution microscopy, video-microscopy, target inhibition applications but also for diagnosis and therapeutic purposes Our optimized screening platform in yeast enables the identification of the protein partners of proteins, DNA sequences, RNA and small molecules of therapeutic interest. Highly complex protein domain libraries, prepared from any cell type or tissue, are screened to saturation to identify partners and their interacting domains using a transcriptional read-out. Sophisticated bioinformatics analysis allows to attribute confidence scores to each interaction. With more than 3,200 academic and industrial customers from over 40 countries, Hybrigenics Services is internationally recognized. Among our services, our yeast two-hybrid screening technology, ULTImate Y2H, is extensively used in many research projects. The results obtained at Hybrigenics have been quoted in more than 480 scientific publications to date. Hybribody offers single-domain antibody selection and validation services. We handle the in vitro selection of antibodies against your protein from a fully synthetic humanized VHH library by an optimized Phage Display technology. VHHs have the advantages of being very small (15 kDa), affine and stable molecules. We validate the selected VHHs for in vitro and in vivo applications (ELISA, IF, Intrabody…). We can also fuse the VHH with Fc fragments from the species of your choice to deliver fully functional recombinant antibodies. In addition, Hybrigenics Services provides optimization of nanobodies coming from the Llama using yeast display technology. Nanobody® is a trademark of Ablynx.

Cambridge, MA, USAHybrigenics Services is a complete service provider of cutting-edge technologies dedicated to the study of proteins and their interactions in 35+ organisms and any research thematic. We offer three main lines of services: • Interactions Discovery: ULTImate Y2H™ to discover novel protein-protein interactions in various tissues/cell types o Several adapted versions allow to identify interactions with transmembrane proteins, DNA and RNA o Associated services: Interaction Domain Mapping, Low binders, super binders • Drug Discovery Services (Adapted from ULTImate Y2H) o Target deconvolution: ULTImate YChemH™ to identify on- and off-targets of small bioactive molecules - mechanism of action, polypharmacology, drug repositioning, prediction of toxicity o Protein-protein interactions induced by molecular glues (protein stabilization, targeted protein degradation) • Nanobody® (or Synthetic Antibodies, VHH) to select and validate high-affinity single-domain antibodies (Hybribody™) - for IF, ELISA, high-resolution microscopy, video-microscopy, target inhibition applications but also for diagnosis and therapeutic purposes Our optimized screening platform in yeast enables the identification of the protein partners of proteins, DNA sequences, RNA and small molecules of therapeutic interest. Highly complex protein domain libraries, prepared from any cell type or tissue, are screened to saturation to identify partners and their interacting domains using a transcriptional read-out. Sophisticated bioinformatics analysis allows to attribute confidence scores to each interaction. With more than 3,200 academic and industrial customers from over 40 countries, Hybrigenics Services is internationally recognized. Among our services, our yeast two-hybrid screening technology, ULTImate Y2H, is extensively used in many research projects. The results obtained at Hybrigenics have been quoted in more than 480 scientific publications to date. Hybribody offers single-domain antibody selection and validation services. We handle the in vitro selection of antibodies against your protein from a fully synthetic humanized VHH library by an optimized Phage Display technology. VHHs have the advantages of being very small (15 kDa), affine and stable molecules. We validate the selected VHHs for in vitro and in vivo applications (ELISA, IF, Intrabody…). We can also fuse the VHH with Fc fragments from the species of your choice to deliver fully functional recombinant antibodies. In addition, Hybrigenics Services provides optimization of nanobodies coming from the Llama using yeast display technology. Nanobody® is a trademark of Ablynx.