1 Røvedvej Trige, 8380 DenmarkBiotest Facility is a Danish contract research organization specializing in biomarker discovery, translational bioanalysis, and preclinical research. The integration of BioXpedia in 2024 combined BioXpedia's established expertise in high-throughput proteomics and biomarker discovery with Biotest Facility's strengths in preclinical pharmacology, creating a uniquely integrated translational CRO. We provide high-quality and cost-effective bioanalytical services for projects ranging from targeted biomarker analyses to comprehensive preclinical studies. Our core expertise spans biomarker discovery and bioanalysis, in vivo pharmacology, molecular biology, bioinformatics, and biostatistics. We utilize technologies including Olink® Proximity Extension Assay (PEA), Meso Scale Discovery (MSD), ELISA, NanoString nCounter®, Fluidigm BioMark HD, digital PCR (dPCR), qPCR, and LC-MS/MS, and work with plasma, serum, CSF, tissue, FFPE, PBMCs, cell lysates, and other biological matrices. The breadth of technologies offered at Biotest Facility enables selection of the most appropriate analytical approach for your project, whether the objective is high- or low-throughput analysis, broad biomarker screening or targeted quantification, while maximizing information obtained from valuable sample material. A unique strength of Biotest Facility is our capability to integrate in vivo pharmacology with LC-MS bioanalysis and advanced biomarker technologies, enabling seamless progression from disease models and efficacy studies to assessment of drug exposure, biological response, and translational endpoints within a single project. Our services are organized across the following core areas of expertise: BIOMARKER DISCOVERY & BIOANALYSIS Biotest Facility specializes in biomarker discovery, biomarker validation, protein profiling, cytokine analysis, and quantitative bioanalysis across a wide range of biological matrices. Our capabilities range from high-throughput, high-multiplex proteomics requiring only minimal sample volumes to targeted low- and mid-plex analyses for biomarker validation and quantitative measurement, using technologies including: • Olink with a wide range of panels including Explore HT, Reveal, Target, and Flex • Meso Scale Discovery (MSD) • ELISA • LC-MS/MS IN VIVO PHARMACOLOGY & DISEASE MODELS Biotest Facility has extensive experience in designing and conducting in vivo studies to support drug discovery and preclinical development. We offer services centered around pharmacokinetics (PK), pharmacodynamics (PD), efficacy testing, adverse clinical symptom assessment, and DMPK investigations in rodent (rat and mice) models. Our expertise spans oncology, immunology, metabolics, neuromuscular disorders, and aging. We collaborate closely with you to ensure that study design, model selection, and endpoint strategies are aligned with the scientific objectives and development stage of your project. A unique strength of Biotest Facility is our capability to extend studies beyond traditional PK/PD and efficacy endpoints through integration with downstream biomarker profiling, gene expression analyses, and LC-MS bioanalysis, allowing a deeper mechanistic or translational insight. Our in vivo models and functional readouts include: Disease Areas & Models: • Oncology: Xenograft models (CDX) and syngeneic models • Immunology & Immunotherapy: Induced autoimmunity models and vaccine studies • Neuromuscular Disorders: Experimental Autoimmune Myasthenia Gravis (EAMG) models and muscular dystrophy models • Metabolic Disease: Diet-Induced Obesity (DIO) models • Aging & Functional Decline: Aging models, longevity, sarcopenia, and lifespan studies • Genetic models Functional Readouts & Downstream Analyses: • Panels of muscle function testing covering strength, balance, endurance, mobility, in vivo force, and grip strength. • Neuromuscular testing including compound muscle action potential (CMAP) • Challenge analyses including metabolic challenge with insulin tolerance testing • Clinical score assessment • Biomarker profiling • Gene expression analysis • Bioanalysis including LC-MS, single-and multiplex immunoassays GENE EXPRESSION & MOLECULAR BIOLOGY Biotest Facility has extensive experience with gene expression profiling, biomarker validation, SNP genotyping, copy number analysis, and molecular characterization for translational and preclinical research. Our workflows support a wide range of sample types, including blood, tissue, PBMCs, fresh frozen material, and FFPE samples, enabling sensitive and scalable molecular analysis across diverse study designs using technologies including: • Bruker (formerly NanoString) nCounter • Fluidigm BioMark HD • Digital PCR (dPCR) • Quantitative PCR (qPCR) SAMPLE PROCESSING & CELL-BASED SERVICES Biotest Facility provides sample preparation, nucleic acid and protein extraction, quality control, primary cell services, and cell-based assays to support downstream analytical workflows and translational research studies. Our dedicated laboratory facilities and automated workflows ensure high-quality sample handling and reproducible experimental results using capabilities including: • RNA extraction • DNA extraction • Protein extraction • FFPE processing • PBMC services and primary cell isolation • Cell culture and expansion • ASO screening • Gymnosis and transfection • Toxicity assays • Cell proliferation assays DATA ANALYSIS & BIOINFORMATICS Biotest Facility offer integrated bioinformatics and biostatistical support to transform complex datasets into biologically meaningful insights. Our data analysis services support biomarker discovery, molecular profiling, translational research, and preclinical studies through capabilities including: • Normal distribution test • Differential expression analysis • Biomarker signature development • Pathway analysis • PCA and clustering • Linear mixed effects analysis • Statistical analysis • Data visualization • Integrated multi-platform analysis

Roevedvej 1 Trige, DK-8380 DenmarkBiotest Facility is a Danish contract research organization specializing in biomarker discovery, translational bioanalysis, and preclinical research. The integration of BioXpedia in 2024 combined BioXpedia's established expertise in high-throughput proteomics and biomarker discovery with Biotest Facility's strengths in preclinical pharmacology, creating a uniquely integrated translational CRO. We provide high-quality and cost-effective bioanalytical services for projects ranging from targeted biomarker analyses to comprehensive preclinical studies. Our core expertise spans biomarker discovery and bioanalysis, in vivo pharmacology, molecular biology, bioinformatics, and biostatistics. We utilize technologies including Olink® Proximity Extension Assay (PEA), Meso Scale Discovery (MSD), ELISA, NanoString nCounter®, Fluidigm BioMark HD, digital PCR (dPCR), qPCR, and LC-MS/MS, and work with plasma, serum, CSF, tissue, FFPE, PBMCs, cell lysates, and other biological matrices. The breadth of technologies offered at Biotest Facility enables selection of the most appropriate analytical approach for your project, whether the objective is high- or low-throughput analysis, broad biomarker screening or targeted quantification, while maximizing information obtained from valuable sample material. A unique strength of Biotest Facility is our capability to integrate in vivo pharmacology with LC-MS bioanalysis and advanced biomarker technologies, enabling seamless progression from disease models and efficacy studies to assessment of drug exposure, biological response, and translational endpoints within a single project. Our services are organized across the following core areas of expertise: BIOMARKER DISCOVERY & BIOANALYSIS Biotest Facility specializes in biomarker discovery, biomarker validation, protein profiling, cytokine analysis, and quantitative bioanalysis across a wide range of biological matrices. Our capabilities range from high-throughput, high-multiplex proteomics requiring only minimal sample volumes to targeted low- and mid-plex analyses for biomarker validation and quantitative measurement, using technologies including: • Olink with a wide range of panels including Explore HT, Reveal, Target, and Flex • Meso Scale Discovery (MSD) • ELISA • LC-MS/MS IN VIVO PHARMACOLOGY & DISEASE MODELS Biotest Facility has extensive experience in designing and conducting in vivo studies to support drug discovery and preclinical development. We offer services centered around pharmacokinetics (PK), pharmacodynamics (PD), efficacy testing, adverse clinical symptom assessment, and DMPK investigations in rodent (rat and mice) models. Our expertise spans oncology, immunology, metabolics, neuromuscular disorders, and aging. We collaborate closely with you to ensure that study design, model selection, and endpoint strategies are aligned with the scientific objectives and development stage of your project. A unique strength of Biotest Facility is our capability to extend studies beyond traditional PK/PD and efficacy endpoints through integration with downstream biomarker profiling, gene expression analyses, and LC-MS bioanalysis, allowing a deeper mechanistic or translational insight. Our in vivo models and functional readouts include: Disease Areas & Models: • Oncology: Xenograft models (CDX) and syngeneic models • Immunology & Immunotherapy: Induced autoimmunity models and vaccine studies • Neuromuscular Disorders: Experimental Autoimmune Myasthenia Gravis (EAMG) models and muscular dystrophy models • Metabolic Disease: Diet-Induced Obesity (DIO) models • Aging & Functional Decline: Aging models, longevity, sarcopenia, and lifespan studies • Genetic models Functional Readouts & Downstream Analyses: • Panels of muscle function testing covering strength, balance, endurance, mobility, in vivo force, and grip strength. • Neuromuscular testing including compound muscle action potential (CMAP) • Challenge analyses including metabolic challenge with insulin tolerance testing • Clinical score assessment • Biomarker profiling • Gene expression analysis • Bioanalysis including LC-MS, single-and multiplex immunoassays GENE EXPRESSION & MOLECULAR BIOLOGY Biotest Facility has extensive experience with gene expression profiling, biomarker validation, SNP genotyping, copy number analysis, and molecular characterization for translational and preclinical research. Our workflows support a wide range of sample types, including blood, tissue, PBMCs, fresh frozen material, and FFPE samples, enabling sensitive and scalable molecular analysis across diverse study designs using technologies including: • Bruker (formerly NanoString) nCounter • Fluidigm BioMark HD • Digital PCR (dPCR) • Quantitative PCR (qPCR) SAMPLE PROCESSING & CELL-BASED SERVICES Biotest Facility provides sample preparation, nucleic acid and protein extraction, quality control, primary cell services, and cell-based assays to support downstream analytical workflows and translational research studies. Our dedicated laboratory facilities and automated workflows ensure high-quality sample handling and reproducible experimental results using capabilities including: • RNA extraction • DNA extraction • Protein extraction • FFPE processing • PBMC services and primary cell isolation • Cell culture and expansion • ASO screening • Gymnosis and transfection • Toxicity assays • Cell proliferation assays DATA ANALYSIS & BIOINFORMATICS Biotest Facility offer integrated bioinformatics and biostatistical support to transform complex datasets into biologically meaningful insights. Our data analysis services support biomarker discovery, molecular profiling, translational research, and preclinical studies through capabilities including: • Normal distribution test • Differential expression analysis • Biomarker signature development • Pathway analysis • PCA and clustering • Linear mixed effects analysis • Statistical analysis • Data visualization • Integrated multi-platform analysis